N-SIM E Super-Resolution Microscope from Nikon Instruments Inc.

Product Specs
ItemN-SIM E Super-Resolution Microscope
CompanyNikon Instruments Inc.
Price Get Pricing Get Quote for this Product
Catalog NumberInquire
QuantityEA
ApplicationsCell Signaling
TypeSuper-Resolution
Resolution85 to 110nm (Lateral (XY)), 250 to 300nm (Axial (Z))
Wavelength(s)488nm / 561nm / 640nm

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Nikon Instruments Inc.

1300 Walt Whitman Road

Melville, New York 11747

United States

Phone: 800-526-4566
1-800-52-NIKON (1-800-526-4566)
Fax: 631-547-0306

Company Profile

Website: https://www.microscope.healthcare.nikon.com/

(1) IdeS, a secreted proteinase of Streptococcus pyogenes, is bound to a nuclease at the bacterial surface where it inactivates opsonizing IgG antibodiesJournal of Biological ChemistryNovember 1, 2023Inga-Maria Frick, Lotta Happonen, Sebastian Wrighton, Pontus Nordenfelt, Lars Björckplaced on top. The samples were allowed to cure overnight before imaging. For image acquisition, a Nikon N-SIM E system with a 100× oil objective (numerical aperture = 1.49) coupled to a Hamamatsu Orca Flash 4.0 camera

(2) Experimental analysis of diverse actin-like proteins from various magnetotactic bacteria by functional expression in Magnetospirillum gryphiswaldensemBioOctober 31, 2023Ram Prasad Awal, Frank D. Müller, Daniel Pfeiffer, Caroline L. Monteil, Guy Perrière, Christopher T. Lefèvre, Dirk Schülerthree-dimensional structured illumination fluorescence microscopy was conducted using an Eclipse Ti2-E N-SIM E fluorescence microscope (Nikon) equipped with a CFI SR Apo TIRF AC 100× H NA1.49 oil objective lens,

(3) Functional expression of foreign magnetosome genes in the alphaproteobacterium Magnetospirillum gryphiswaldensemBioAugust 31, 2023Ram Prasad Awal, Christopher T. Lefevre, Dirk Schülerfusion proteins, Structured Illumination Fluorescent microscopy (SIM) was performed on an Eclipse Ti2-E N-SIM E fluorescence microscope (Nikon) equipped with a CFI SR Apo TIRF AC 100× H NA1.49 Oil objective lens,

(4) Controllable adhesive mechanisms via the internal fibers in soft footpads of honeybeesMaterials Today BioAugust 1, 2023Lulu Liang, Jieliang Zhao, Qun Niu, Li Yu, Zhiyun Ma, Xiangbing Wu, Wenzhong Wang, Shaoze Yancoverslip. Subsequently, the sample was examined with the confocal laser scanning microscopy system (N-SIM E, Nikon, Japan) equipped with four lasers (laser lines; 405 nm, blue; 488 nm, green; 561 nm, yellow

(5) Acetylated α-Tubulin and α-Synuclein: Physiological Interplay and Contribution to α-Synuclein OligomerizationInt J Mol SciJuly 31, 2023Alessandra Maria Calogero, Milo Jarno Basellini, Huseyin Berkcan Isilgan, Francesca Longhena, Arianna Bellucci, Samanta Mazzetti, Chiara Rolando, Gianni Pezzoli, Graziella Cappellettiwhile “analyze particles” was used for the AS-PLA puncta analyses. For super-resolution images, the N-SIM E structured light super-resolution module was used coupled with a 100× oil-immersion objectives (512

(6) SuPAR mediates viral response proteinuria by rapidly changing podocyte functionNature communicationsJuly 21, 2023Wei C, Datta PK, Siegerist F, Li J, Yashwanth S, Koh KH, Kriho NW, Ismail A, Luo S, Fischer T, et al.PBS wash. For 3D SIM, z-stacks of 21 planes of both channels (488 and 561 nm) were acquired using the N-SIM-E super-resolution microscope (Nikon) equipped with a ×100 silicone objective. The images were reconstructed into 3D SIM images using

(7) Exploring the ATR-CHK1 pathway in the response of doxorubicin-induced DNA damages in acute lymphoblastic leukemia cellsCell Biology and ToxicologyJune 1, 2023Andrea Ghelli Luserna Di Rorà, Martina Ghetti, Lorenzo Ledda, Anna Ferrari, Matteo Bocconcelli, Antonella Padella, Roberta Napolitano, Maria Chiara Fontana, Chiara Liverani, Enrica Imbrogno, et al.three times in 1X PBS and mounted using ProLong Antifade DAPI (Invitrogen). Cells were imaged with a N-SIM E laser confocal microscope (Nikon Corporation) at a magnification of 60× and analyzed with NIS Elements

(8) Interacting bactofilins impact cell shape of the MreB-less multicellular Rhodomicrobium vannieliiPLOS GeneticsMay 31, 2023Pia Richter, Brigitte Melzer, Frank D. Müllerconstructs and epifluorescence of strains labelled with two fluorophores was performed on an Eclipse Ti2-E N-SIM E fluorescence microscope (Nikon) equipped with a CFI SR Apo TIRF AC 100×H NA1.49 oil objective lens,

(9) Mapping rhodopsin trafficking in rod photoreceptors with quantitative super-resolution microscopybioRxiv : the preprint server for biologyApril 21, 2023Haggerty KN, Eshelman SC, Sexton LA, Frimpong E, Rogers LM, Agosto MA, Robichaux MA.1xPBS, mounted in ProLong Glass Antifade Mountant, and cured for 2 days at RT before SIM. SIM. A Nikon N-SIM E microscope system equipped with a Hamamatsu Orca-Flash 4.0 camera was used for imaging at RT with a

(10) Tetramerization of STAT5 regulates monocyte differentiation and the dextran sulfate sodium-induced colitis in miceFrontiers in ImmunologyApril 20, 2023Kelly L. Monaghan, Wen Zheng, Halima Akhter, Lei Wang, Amanda G. Ammer, Peng Li, Jian-Xin Lin, Gangqing Hu, Warren J. Leonard, Edwin C. K. Wanimages from anti-arginase I antibody-stained sections were collected on the Nikon A1R confocal with N-SIM E microscope with the PlanFluor 40x/0.75 objective. Images were generated using NIS Elements software.

(11) CRK2 controls cytoskeleton morphogenesis in Trypanosoma brucei by phosphorylating β-tubulin to regulate microtubule dynamicsPLOS PathogensMarch 22, 2023Kyu Joon Lee, Qing Zhou, Ziyin LiCy3-conjugated anti-rat IgG. Cytoskeletons were imaged under the Nikon Super Resolution Microscope n-SIM E instrument (Nikon Instruments Inc., Americas) with a 100× lens equipped with 488 nm and 592 nm lasers.

(12) Silent gene clusters encode magnetic organelle biosynthesis in a non-magnetotactic phototrophic bacteriumThe ISME JournalMarch 1, 2023M. V. Dziuba, A. Paulus, L. Schramm, R. P. Awal, M. Pósfai, C. L. Monteil, S. Fouteau, R. Uebe, D. Schüleragarose pads. 3D-SIM (striped illumination at 3 angles and 5 phases) was performed on an Eclipse Ti2-E N-SIM E fluorescence microscope (Nikon) equipped with a CFI SR Apo TIRF AC 100×H NA1.49 Oil objective lens,

(13) Enhanced liquidity of p62 droplets mediated by Smurf1 links Nrf2 activation and autophagyCell & bioscienceFebruary 21, 2023Xia Q, Li Y, Xu W, Wu C, Zheng H, Liu L, Dong L.(FRAP) Cells were grown in glass base dishes. FRAP assay was conducted using the FRAP module of the NIKON N-SIM E confocal microscopy system. Proteins with fluorescent label GFP and RFP were bleached using a 488 nm

(14) Synergistic hydroxyl radical formation, system XC- inhibition and heat shock protein crosslinking tango in ferrotherapy: A prove-of-concept study of “sword and shield” theoryMaterials Today BioDecember 1, 2022Li Xie, Wenjie Chen, Qifang Chen, Yang Jiang, Erqun Song, Xiaokang Zhu, Yang SongPBS for three times. After that, cells were imaged under a confocal laser scanning microscope (CLSM, N-SIM E, Nikon). 2.10 In vitro cytotoxicity test For cell viability study, 4T1 cells were seeded in 96-well

(15) The autophagy inducer SMER28 attenuates microtubule dynamics mediating neuroprotectionScientific reportsOctober 25, 2022Kirchenwitz M, Stahnke S, Grunau K, Melcher L, van Ham M, Rottner K, Steffen A, Stradal TEB.live-cell experiments. For SIM (3D structured illumination microscopy) images shown in Figs. 3 and 4, an N-SIM E (Nikon) was used, built on a Ti-Eclipse microscope (Nikon). Data were acquired using a z Piezo drive

(16) Anionic nanoplastic exposure induces endothelial leakinessNature communicationsAugust 13, 2022Wei W, Li Y, Lee M, Andrikopoulos N, Lin S, Chen C, Leong DT, Ding F, Song Y, Ke PC.morphological features of the cells were captured using a confocal fluorescence microscope (Nikon, N-SIM E). Confocal fluorescence microscopy HUVECs (5 × 105 cells/well) were seeded on 8 well chamber slides

(17) A Spef1-interacting microtubule quartet protein in Trypanosoma brucei promotes flagellar inheritance by regulating basal body segregationJournal of Biological ChemistryJuly 1, 2022Kieu T.M. Pham, Qing Zhou, Kyu Joon Lee, Ziyin Lidescribed previously, except that the cells were viewed under the Nikon Super Resolution Microscope n-SIM E instrument (Nikon Instruments Inc). The acquired SIM images were analyzed by the NIS-Elements AR software

(18) A specific EMC subunit supports Dengue virus infection by promoting virus membrane fusion essential for cytosolic genome deliveryPLoS pathogensJuly 1, 2022Bagchi P, Speckhart K, Kennedy A, Tai AW, Tsai B.immersion objective (N.A. 1.4), Sola lumencore light engine and Photometrics CoolSnap HQ camera; or Nikon N-SIM E in 3D-SIM mode with CFI SR HP Apochromat TIRF 100XC Oil immersion objective (NA 1.49), LU-NV series

(19) ScoMorphoFISH: A deep learning enabled toolbox for single-cell single-mRNA quantification and correlative (ultra-)morphometryJournal of cellular and molecular medicineJune 1, 2022Siegerist F, Hay E, Dikou JS, Pollheimer M, Büscher A, Oh J, Ribback S, Zimmermann U, Bräsen JH, Lenoir O, et al.3D‐structured illumination microscopy, a Zeiss Elyra PS.1 system (Carl Zeiss Microsystems) or a Nikon N‐SIM‐E was used as described before. 3 Whole‐slide images of PAS‐stained sections were acquired on a Leica

(20) Shielding of actin by the endoplasmic reticulum impacts nuclear positioningNature communicationsMay 19, 2022Janota CS, Pinto A, Pezzarossa A, Machado P, Costa J, Campinho P, Franco CA, Gomes ER.snapshots (LSM 880 and SIM). SIM microscopy experiments were performed using the ×100 objective of Nikon N-SIM E at 37 °C and cells were treated with HEPES at 20 mM to stabilize the pH during acquisition. Image analysis

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